Science with J

🧬 Do you need an expression plasmid that is not commercially available (e.g. a mutated variant)? 😏 Whether you want the plasmid to contain a mutated gene or a wild-type coding sequence, here is a quick step-by-step guide.📋 The first thing is to find the coding sequence of the wild gene that expresses the protein you want. To do this, go to  https://www.ncbi.nlm.nih.gov/ and search the name of your interest gene using the Nucleotide search. Then click on the desired organism and select  mRNA . Choose the best entry for your query according to your requirement, and select the CDS  (and the protein sequence will appear on the right, a.k.a., translation ) .   Next,  copy-paste both FASTA text codes into a Word file . We will need them later. 👉If you only want to express the wild-type protein... Paste the CDS directly into the corresponding ORF item from the supplier (e.g.  VectorBuilder ).  https://en.vectorbuilder.com/design/pRP_Exp.html You wil...

Systematic reviews The advice that nobody taught you 👂 If you are about to embark on the world of systematic reviews, you will probably have flooded your mind with information from courses that are not at all practical. This is because nothing can replace better learning how to conduct a systematic review than getting down to business. If you follow the traditional method of analysis, you will have to keep trying and failing with your search algorithm , until you get a number of articles that are feasible for you or your team to analyze . To do this, start by asking yourself an unexplored PICO ( P atient/Population; I ntervention; C omparison; O utcomes)  question , and perform a search in one or more databases, using linked terms following the guidelines of boolean logic (AND, OR...) and parentheses. But I've gotten thousands of results!😯 After your boolean search , and after filtering out duplicate publications and articles you missed that do not meet your requirements for...