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Showing posts with the label Biomedicine

Carbon atom ball diagrams in metabolomics experiments

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Represent carbon atoms labeled in metabolic pathways If you've ever wondered how to create those metabolic maps that show the number of atoms labeled by a carbon isotope ( e.g., C-13 ), we'll show you how. But before you begin, you need to know a few important details. https://www.nature.com/articles/s41467-024-52538-5?fromPaywallRec=false What is the Exchange Rate? The Exchange Rate is a parameter that indicates the number of atoms exchanged between the uniformly carbon-labeled molecule to which your cells were initially exposed and the remaining fully or partially labeled metabolites detected in the Isotopic Profiling experiment, also called Isotopic Trace Analysis . In articles, the Average Exchange Rate is often represented in diagrams with filled or empty balls, with the filled balls representing the average number of labeled carbon atoms in the metabolite in question and the empty balls representing the average number of unlabeled carbon atoms for the same metabolite....

Transform RCF/g to RPM in your centrifuge

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Convert RPM to RCF easily💫 Can your centrifuge only be programmed in RPM? Here's a quick solution to find out its equivalent in RCF (also called g). 📝 If you're used to reading scientific papers, you'll have noticed that the typical way of expressing the speed at which you should set a centrifuge is "RCF" or "g". This is because it's a standardized way of referring to the speed at which a centrifuge's rotor spins. What is special about this way of expressing rotational speed? What makes RCF the most accurate way to express the speed at which the centrifuge is set is that this value does not depend on the distance of the tube from the rotor axis, whereas RPM does. That is why we say that RCF is a standardized or normalized parameter. And what is the impact of the RCF being a standardized unit?🧪  By using RCF instead of RPM, you ensure that the centrifugal force you apply to your sample tubes is the same across all centrifuges in the world.🌎🌍 ...

Systematic reviews: How many articles to review?

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Systematic reviews The advice that nobody taught you 👂 If you are about to embark on the world of systematic reviews, you will probably have flooded your mind with information from courses that are not at all practical. This is because nothing can replace better learning how to conduct a systematic review than getting down to business. If you follow the traditional method of analysis, you will have to keep trying and failing with your search algorithm , until you get a number of articles that are feasible for you or your team to analyze . To do this, start by asking yourself an unexplored PICO ( P atient/Population; I ntervention; C omparison; O utcomes)  question , and perform a search in one or more databases, using linked terms following the guidelines of boolean logic (AND, OR...) and parentheses. But I've gotten thousands of results!😯 After your boolean search , and after filtering out duplicate publications and articles you missed that do not meet your requirements for...